Premier service: KASP on Demand
For KASP on Demand (KOD) our scientists design and fully validate and optimize your assay prior to delivery. Any custom KASP genotyping assay can be provided with our validation service ensuring that you receive a working assay.
If you are working with human DNA samples, KOD assay validation can be performed using our validation panel of 44 DNA samples. We can use your own human DNA samples for validation if you prefer, and this is recommended if you are working on an assay with a low minor allele frequency. For all other organisms, LGC require the customer to send in sample DNA to enable assay validation to be carried out in our laboratories. We require a minimum of 22 DNA samples for validation, and will accept up to 94 DNA samples. For more information about how much DNA is required, please see our factsheet.
KASP assays have an industry-leading conversion rate. We have carried out the design for millions of individual assays and we have significant experience in the design of successful assays for even the most difficult genomes or target sequences. With our validation service we guarantee delivery of a working assay or you will not be charged.
Standard service: KASP by Design
KASP by Design (KBD) consists of three KASP primers that are specific to the SNP or InDel of interest. Assay design (primer selection) is carried out using our proprietary Kraken™ software system. Please note that using in-silico validated primers cannot guarantee a working assay. The success rate for custom KASP primer design depends on a number of factors including DNA quality, genome complexity, homology and genome size. Any custom KASP assay can be provided with our additional assay validation service to ensure a working assay.
KASP Genotyping Libraries
Our KASP™ genotyping libraries consist of pre-validated genotyping assays. In collaboration with scientific partners across the globe, we have developed a number of genotyping libraries for various species. We are pleased to enable access to these valuable resources for the genotyping community. Our libraries include:
Human exome sample tracking panel – developed in conjunction with the University of Southampton. A set of 24 thoroughly validated human SNP assays for robust and convenient sample tracking and data validation in sequencing laboratories.
Wheat genotyping library – developed in conjunction with the University of Bristol, and the Integrated Breeding Platform (IBP). This library contains over 8,000 functionally validated SNP assays that are used by researchers and plant breeders to identify genetic markers associated with key phenotypes and traits, to enable Marker Assisted Selection (MAS).
Maize genotyping library – over 1,200 maize SNP assays, developed for CIMMYT’s Global Maize Program and the Generation Challenge Programme.
Soybean genotyping library – over 1,000 soybean SNP assays, developed for the Generation Challenge Programme and the Integrated Breeding Platform.
Rice genotyping library – over 2,000 rice SNP assays, developed for the Generation Challenge Programme and the Integrated Breeding Platform.
Tomato genotyping library – over 7,000 tomato SNP assays, developed in conjunction with the Solanaceae Coordinated Agricultural Project (SolCAP).
Lentil genotyping panel – a panel of 146 validated lentil SNP assays developed in conjunction with the Pulse Crop Research Group at the University of Saskatchewan
Mouse genotyping library – developed in conjunction with the Jackson Laboratory. The mouse panel consists of 1,638 pre-validated assays for mouse genotyping. These markers have been genotyped across many common laboratory mouse strains.
Rat genotyping library – developed in conjunction with the Hubrecht Institute. The rat library offers a set of 820 pre-validated assays for rat genotyping. These assays are powerful tool for the mapping of traits within rat disease models and breeding populations.
Zebrafish genotyping library – developed in conjunction with the Hubrecht Institute and the Wellcome Trust Sanger Institute as part of the Zebrafish Mutation Project. LGC offers a set of 1,073 functionally validated KASP genotyping assays for zebrafish.
KASP Master Mix
Patented, PCR based KASP genotyping technology has been a critical tool for plant and livestock breeders for the last fifteen years, and the ability to screen KASP markers has underpinned breeding programs at industry leading AgBio companies. In the human genotyping world, KASP markers have been used in large cohort research studies to understand human health and disease.
As the AgBio industry moves towards looking at larger numbers of markers in different plant and animal species, and human health research learns more about the genetics underpinning common diseases, scientists at LGC continue to develop improved genotyping tools compatible with KASP technology.
We use KASP Master mix in our own, largest genotyping lab in Europe, so we can troubleshoot any KASP issues in house with our experts using the same instruments as our customers, and bring assays in house at times of peak demand or customer laboratory failures.
Key features of KASP master mix supply and production
Each batch of KASP Master mix is functionally tested in high throughput genotyping
Our products are patent protected, and have undergone continuous development to incorporate customer performance feedback to arrive at the best ever KASP formulation
Our stable supply chain includes disaster recovery processes, allowing us to reliably supply large volumes of KASP Master mix to large, commercial customers
Global teams of sales, customer service and technical support specialists, provide regional support for your product
Our complete workflow solutions allow us to offer instruments, software, and upstream and downstream technologies that are developed with KASP in mind, with fully validated protocols